From: Physicochemical modelling of the retention mechanism of temperature-responsive polymeric columns for HPLC through machine learning algorithms
Mobile phase
Water + 0.1% v/v FA
Flow rate
0.7 mL \(\hbox {min}^{-1}\)
Injection volume
5 \(\upmu \)L
Column temperature
5 and \(45\,^{\circ }\)C