Table 3 Effects of mutations generated by SSP on the thermostability of cold shock protein validated against the collated FireProtDB dataset [38]
From: Anticipating protein evolution with successor sequence predictor
Mutation by SSP | Mean ΔΔG (kcal/mol) | △Tm (°C)a | Prediction agreement type |
|---|---|---|---|
L2R | + 0.4 | N/A | Site agreeing |
E3K | − 2.48 | + 16.6 | Mutation agreeing |
E3Q | − 1.09 | + 7.3 | Mutation agreeing |
E3R | − 1.65 | + 16.0 | Site agreeing |
E3V | − 1.8 | N/A | Site agreeing |
D24N | + 0.66 | − 6.9 | Site agreeing |
A46E | + 0.07 | − 5.0 | Site agreeing |
A46K | − 1.41 | + 8.4 | Site agreeing |
A46L | − 0.8 | N/A | Site agreeing |
E50K | + 0.33 | − 5.6 | Site agreeing |
N55D | − 0.46 | + 3.9 | Site agreeing |
N55K | 0 | + 0.8 | Mutation agreeing |
N55S | + 0.2 | N/A | Site agreeing |
E66K | − 2.17 | + 12.9 | Site agreeing |